3PU1: Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyG complex

Citation:
Abstract
The genomic RNA of negative-strand RNA viruses, such as vesicular stomatitis virus (VSV), is completely enwrapped by the nucleocapsid protein (N) in every stage of virus infection. During viral transcription/replication, however, the genomic RNA in the nucleocapsid must be accessible by the virus-encoded RNA-dependent RNA polymerase in order to serve as the template for RNA synthesis. With the VSV nucleocapsid and a nucleocapsid-like particle (NLP) produced in Escherichia coli, we have found that the RNA in the VSV nucleocapsid can be removed by RNase A, in contrast to what was previously reported. Removal of the RNA did not disrupt the assembly of the N protein, resulting in an empty capsid. Polyribonucleotides were reencapsidated into the empty NLP, and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence.
PDB ID: 3PU1Download
MMDB ID: 87925
PDB Deposition Date: 2010/12/3
Updated in MMDB: 2011/05
Experimental Method:
x-ray diffraction
Resolution: 3.14  Å
Source Organism:
Vesicular stomatitis Indiana virus
Similar Structures:
Biological Unit for 3PU1: dodecameric; determined by author and by software (PISA)
Molecular Components in 3PU1
Label Count Molecule
Proteins (10 molecules)
10
Nucleoprotein
Molecule annotation
Nucleotide(1 molecule)
2
RNA (45-mer)
Molecule annotation
Chemicals (36 molecules)
1
36
* Click molecule labels to explore molecular sequence information.

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