3PAW: Low Resolution X-Ray Crystal Structure Of Yeast Rnr1p With Datp Bound In The A-Site

Citation:
Abstract
Ribonucleotide reductase (RR) is an alpha(n)beta(n) (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the alpha(6)-betabeta'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.
PDB ID: 3PAWDownload
MMDB ID: 88840
PDB Deposition Date: 2010/10/19
Updated in MMDB: 2011/05
Experimental Method:
x-ray diffraction
Resolution: 6.61  Å
Source Organism:
Similar Structures:
Biological Unit for 3PAW: dimeric; determined by software (PISA)
Molecular Components in 3PAW
Label Count Molecule
Proteins (2 molecules)
2
Ribonucleoside-diphosphate Reductase Large Chain 1(Gene symbol: RNR1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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