3PA7: Crystal Structure Of Fkbp From Plasmodium Vivax In Complex With Tetrapeptide Alpf

Citation:
Abstract
The immunosuppressive drug FK506 binding proteins (FKBPs), an immunophilin family with the immunosuppressive drug FK506 binding property, exhibit peptidylprolyl cis-trans isomerase (PPIase) activity. While the cyclophilin-catalyzed peptidylprolyl isomerization of X-Pro peptide bonds has been extensively studied, the mechanism of the FKBP-mediated peptidylprolyl isomerization remains uncharacterized. Thus, to investigate the binding of FKBP with its substrate and the underlying catalytic mechanism of the FKBP-mediated proline isomerization, here we employed the FK506 binding domain (FKBD) of the human malarial parasite Plasmodium vivax FK506 binding protein 35 (PvFKBP35) and examined the details of the molecular interaction between the isomerase and a peptide substrate. The crystallographic structures of apo PvFKBD35 and its complex with the tetrapeptide substrate succinyl-Ala-Leu-Pro-Phe-p-nitroanilide (sALPFp) determined at 1.4 A and 1.65 A resolutions, respectively, showed that the substrate binds to PvFKBD35 in a cis conformation. Nuclear magnetic resonance (NMR) studies demonstrated the chemical shift perturbations of D55, H67, V73, and I74 residues upon the substrate binding. In addition, the X-ray crystal structure, along with the mutational studies, shows that Y100 is a key residue for the catalytic activity. Taken together, our results provide insights into the catalytic mechanism of PvFKBP35-mediated cis-trans isomerization of substrate and ultimately might aid designing substrate mimetic inhibitors targeting the malarial parasite FKBPs.
PDB ID: 3PA7Download
MMDB ID: 97562
PDB Deposition Date: 2010/10/18
Updated in MMDB: 2013/06
Experimental Method:
x-ray diffraction
Resolution: 2.61  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3PA7: trimeric; determined by author
Molecular Components in 3PA7
Label Count Molecule
Proteins (3 molecules)
2
70 KDA Peptidylprolyl Isomerase, Putative
Molecule annotation
1
4-mer Peptide Alpf
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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