3P8U: Crystal Structure Of Meosfp In Its Green State

Citation:
Abstract
Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores with controllable emission properties. Chief among these fluorophores are the photoactivatable fluorescent proteins capable of reversible on/off photoswitching or irreversible green-to-red photoconversion. IrisFP was recently reported as the first fluorescent protein combining these two types of phototransformations. The introduction of this protein resulted in new applications such as super-resolution pulse-chase imaging. However, the spectroscopic properties of IrisFP are far from being optimal and its tetrameric organization complicates its use as a fusion tag. Here, we demonstrate how four-state optical highlighting can be rationally introduced into photoconvertible fluorescent proteins and develop and characterize a new set of such enhanced optical highlighters derived from mEosFP and Dendra2. We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic properties that make it ideal for advanced fluorescence-based imaging applications.
PDB ID: 3P8UDownload
MMDB ID: 94435
PDB Deposition Date: 2010/10/15
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 2.25  Å
Source Organism:
Similar Structures:
Biological Unit for 3P8U: monomeric; determined by author
Molecular Components in 3P8U
Label Count Molecule
Protein (1 molecule)
1
Green to RED Photoconvertible Gpf-like Protein Eosfp
Molecule annotation
Chemicals (8 molecules)
1
2
2
6
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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