3OXJ: Crystal Structure Of Glycine Riboswitch, Soaked In Ba2+

Citation:
Abstract
Glycine riboswitches regulate gene expression by feedback modulation in response to cooperative binding to glycine. Here, we report on crystal structures of the second glycine-sensing domain from the Vibrio cholerae riboswitch in the ligand-bound and unbound states. This domain adopts a three-helical fold that centers on a three-way junction and accommodates glycine within a bulge-containing binding pocket above the junction. Glycine recognition is facilitated by a pair of bound Mg(2+) cations and governed by specific interactions and shape complementarity with the pocket. A conserved adenine extrudes from the binding pocket and intercalates into the junction implying that glycine binding in the context of the complete riboswitch could impact on gene expression by stabilizing the riboswitch junction and regulatory P1 helix. Analysis of riboswitch interactions in the crystal and footprinting experiments indicates that adjacent glycine-sensing modules of the riboswitch could form specific interdomain interactions, thereby potentially contributing to the cooperative response.
PDB ID: 3OXJDownload
MMDB ID: 87641
PDB Deposition Date: 2010/9/21
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 3.2  Å
Biological Unit for 3OXJ: monomeric; determined by author
Molecular Components in 3OXJ
Label Count Molecule
Nucleotide(1 molecule)
1
Domain II of Glycine Riboswitch
Molecule annotation
Chemicals (22 molecules)
1
1
2
13
3
8
* Click molecule labels to explore molecular sequence information.

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