3OV7: How The Cca-Adding Enzyme Selects Adenine Over Cytosine In Position 76 Of Trna

Citation:
Abstract
CCA-adding enzymes [ATP(CTP):tRNA nucleotidyltransferases] add CCA onto the 3' end of transfer RNA (tRNA) precursors without using a nucleic acid template. Although the mechanism by which cytosine (C) is selected at position 75 of tRNA has been established, the mechanism by which adenine (A) is selected at position 76 remains elusive. Here, we report five cocrystal structures of the enzyme complexed with both a tRNA mimic and nucleoside triphosphates under catalytically active conditions. These structures suggest that adenosine 5'-monophosphate is incorporated onto the A76 position of the tRNA via a carboxylate-assisted, one-metal-ion mechanism with aspartate 110 functioning as a general base. The discrimination against incorporation of cytidine 5'-triphosphate (CTP) at position 76 arises from improper placement of the alpha phosphate of the incoming CTP, which results from the interaction of C with arginine 224 and prevents the nucleophilic attack by the 3' hydroxyl group of cytidine75.
PDB ID: 3OV7Download
MMDB ID: 86652
PDB Deposition Date: 2010/9/15
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Archaeoglobus fulgidus
Similar Structures:
Biological Unit for 3OV7: tetrameric; determined by author and by software (PISA)
Molecular Components in 3OV7
Label Count Molecule
Proteins (2 molecules)
2
Cca-adding Enzyme(Gene symbol: AF_RS10845)
Molecule annotation
Nucleotide(1 molecule)
2
RNA (34-mer)
Molecule annotation
Chemicals (6 molecules)
1
2
2
2
3
1
4
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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