3OGM: Structure Of Coi1-Ask1 In Complex With Coronatine And The Jaz1 Degron

Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved alpha-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.
PDB ID: 3OGMDownload
MMDB ID: 85512
PDB Deposition Date: 2010/8/17
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 3.34  Å
Source Organism:
Similar Structures:
Biological Unit for 3OGM: trimeric; determined by author and by software (PISA)
Molecular Components in 3OGM
Label Count Molecule
Proteins (3 molecules)
Skp1-like Protein 1A(Gene symbol: SKP1)
Molecule annotation
Coronatine-insensitive Protein 1(Gene symbol: COI1)
Molecule annotation
Jaz1 Degron Peptide
Molecule annotation
Chemicals (5 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB