3O7A: Crystal Structure Of Phf13 In Complex With H3k4me3

PHF13 is a chromatin affiliated protein with a functional role in differentiation, cell division, DNA damage response and higher chromatin order. To gain insight into PHF13's ability to modulate these processes, we elucidate the mechanisms targeting PHF13 to chromatin, its genome wide localization and its molecular chromatin context. Size exclusion chromatography, mass spectrometry, X-ray crystallography and ChIP sequencing demonstrate that PHF13 binds chromatin in a multivalent fashion via direct interactions with H3K4me2/3 and DNA, and indirectly via interactions with PRC2 and RNA PolII. Furthermore, PHF13 depletion disrupted the interactions between PRC2, RNA PolII S5P, H3K4me3 and H3K27me3 and resulted in the up and down regulation of genes functionally enriched in transcriptional regulation, DNA binding, cell cycle, differentiation and chromatin organization. Together our findings argue that PHF13 is an H3K4me2/3 molecular reader and transcriptional co-regulator, affording it the ability to impact different chromatin processes.
PDB ID: 3O7ADownload
MMDB ID: 85374
PDB Deposition Date: 2010/7/30
Updated in MMDB: 2010/10
Experimental Method:
x-ray diffraction
Resolution: 1.67  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 3O7A: dimeric; determined by software (PISA)
Molecular Components in 3O7A
Label Count Molecule
Proteins (2 molecules)
PHD Finger Protein 13 Variant
Molecule annotation
H3k4me3 Histone 11mer-peptide
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB