3O62: Nucleosome Core Particle Modified With A Cisplatin 1,3-Cis-{pt(Nh3) 2}2+-D(Gptpg) Intrastrand Cross-Link

Citation:
Abstract
The effects of cisplatin binding to DNA were explored at the nucleosome level to incorporate key features of the eukaryotic nuclear environment. An X-ray crystal structure of a site-specifically platinated nucleosome carrying a 1,3-cis-{Pt(NH)}(2)+-d(GpTpG) intrastrand cross-link reveals the details of how this adduct dictates the rotational positioning of DNA in the nucleosome. Results from in vitro nucleosome mobility assays indicate that a single platinum adduct interferes with ATP-independent sliding of DNA around the octamer core. Data from in vitro transcription experiments suggest that RNA polymerases can successfully navigate along cisplatin-damaged DNA templates that contain nucleosomes, but stall when the transcription elongation complex physically contacts a platinum cross-link located on the template strand. These results provide information about the effects of cisplatin binding to nuclear DNA and enhance our understanding of the mechanism of transcription inhibition by platinum anticancer compounds.
PDB ID: 3O62Download
MMDB ID: 87713
PDB Deposition Date: 2010/7/28
Updated in MMDB: 2011/05
Experimental Method:
x-ray diffraction
Resolution: 3.22  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3O62: decameric; determined by author and by software (PISA)
Molecular Components in 3O62
Label Count Molecule
Proteins (8 molecules)
2
Histone H3.2(Gene symbol: hist2h3c.L)
Molecule annotation
2
Histone H4(Gene symbol: hist1h4a.L)
Molecule annotation
2
Histone H2A Type 1(Gene symbol: h2afx.L)
Molecule annotation
2
Histone H2B 1.1(Gene symbol: hist1h2bj.S)
Molecule annotation
Nucleotides(2 molecules)
1
DNA (146-mer)
Molecule annotation
1
DNA (146-mer)
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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