3NYG: X-Ray Structure Of Ester Chemical Analogue [o-Gly51,O-Gly51']hiv-1 Protease Complexed With Mvt-101 Inhibitor

We have used chemical protein synthesis and advanced physical methods to probe dynamics-function correlations for the HIV-1 protease, an enzyme that has received considerable attention as a target for the treatment of AIDS. Chemical synthesis was used to prepare a series of unique analogues of the HIV-1 protease in which the flexibility of the "flap" structures (residues 37-61 in each monomer of the homodimeric protein molecule) was systematically varied. These analogue enzymes were further studied by X-ray crystallography, NMR relaxation, and pulse-EPR methods, in conjunction with molecular dynamics simulations. We show that conformational isomerization in the flaps is correlated with structural reorganization of residues in the active site, and that it is preorganization of the active site that is a rate-limiting factor in catalysis.
PDB ID: 3NYGDownload
MMDB ID: 94745
PDB Deposition Date: 2010/7/15
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 1.45  Å
Source Organism:
Similar Structures:
Biological Unit for 3NYG: dimeric; determined by author and by software (PISA)
Molecular Components in 3NYG
Label Count Molecule
Proteins (2 molecules)
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB