National Center for
3M67: Crystal Structure Of Human Carbonic Anhydrase Isozyme Ii With 2- Chloro-5-[(6,7-Dihydro-1h-[1,4]dioxino[2,3-F]benzimidazol-2- Ylsulfanyl)acetyl]benzenesulfonamide
Bioorg. Med. Chem. (2010) 18 p.7357-7364
A series of novel 2-chloro-5-[(1-benzimidazolyl- and 2-benzimidazolylsulfanyl)acetyl]benzene-sulfonamides were designed and synthesized. Their binding to recombinant human carbonic anhydrase (hCA) isozymes I, II, VII, and XIII was determined by isothermal titration calorimetry and thermal shift assay. The designed S-alkylated benzimidazole derivatives exhibited stronger binding than the indapamide-like N-alkylated benzimidazoles, with the K(d) reaching about 50-100 nM with drug-targeted hCAs VII and XIII. The cocrystal structures of selected compounds with hCA II were determined by X-ray crystallography, and structural features of the binding event were revealed.