3LIK: Human Mmp12 In Complex With Non-Zinc Chelating Inhibitor

Citation:
Abstract
After the disappointment of clinical trials with early broad spectrum synthetic inhibitors of matrix metalloproteinases (MMPs), the field is now resurging with a new focus on the development of selective inhibitors that fully discriminate between different members of the MMP family with several therapeutic applications in perspective. Here, we report a novel class of highly selective MMP-12 inhibitors, without a phosphinic zinc-binding group, designed to plunge deeper into the S(1)' cavity of the enzyme. The best inhibitor from this series, identified through a systematic chemical exploration, displays nanomolar potency toward MMP-12 and selectivity factors that range between 2 and 4 orders of magnitude toward a large set of MMPs. Comparison of the high resolution x-ray structures of MMP-12 in free state or bound to this new MMP-12 selective inhibitor reveals that this compound fits deeply within the S(1)' specificity cavity, maximizing surface/volume ratios, without perturbing the S(1)' loop conformation. This is in contrast with highly selective MMP-13 inhibitors that were shown to select a particular S(1)' loop conformation. The search for such compounds that fit precisely to preponderant S(1)' loop conformation of a particular MMP may prove to be an alternative effective strategy for developing selective inhibitors of MMPs.
PDB ID: 3LIKDownload
MMDB ID: 84551
PDB Deposition Date: 2010/1/25
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 3LIK: monomeric; determined by author and by software (PISA)
Molecular Components in 3LIK
Label Count Molecule
Protein (1 molecule)
1
Macrophage Metalloelastase(Gene symbol: MMP12)
Molecule annotation
Chemicals (10 molecules)
1
2
2
3
3
1
4
1
5
3
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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