3LHD: Crystal Structure Of P. Abyssi Trna M1a58 Methyltransferase In Complex With S-Adenosyl-L-Homocysteine

Citation:
Abstract
The S-adenosyl-L-methionine dependent methylation of adenine 58 in the T-loop of tRNAs is essential for cell growth in yeast or for adaptation to high temperatures in thermophilic organisms. In contrast to bacterial and eukaryotic tRNA m(1)A58 methyltransferases that are site-specific, the homologous archaeal enzyme from Pyrococcus abyssi catalyzes the formation of m(1)A also at the adjacent position 57, m(1)A57 being a precursor of 1-methylinosine. We report here the crystal structure of P. abyssi tRNA m(1)A57/58 methyltransferase ((Pab)TrmI), in complex with S-adenosyl-L-methionine or S-adenosyl-L-homocysteine in three different space groups. The fold of the monomer and the tetrameric architecture are similar to those of the bacterial enzymes. However, the inter-monomer contacts exhibit unique features. In particular, four disulfide bonds contribute to the hyperthermostability of the archaeal enzyme since their mutation lowers the melting temperature by 16.5 degrees C. His78 in conserved motif X, which is present only in TrmIs from the Thermococcocales order, lies near the active site and displays two alternative conformations. Mutagenesis indicates His78 is important for catalytic efficiency of (Pab)TrmI. When A59 is absent in tRNA(Asp), only A57 is modified. Identification of the methylated positions in tRNAAsp by mass spectrometry confirms that (Pab)TrmI methylates the first adenine of an AA sequence.
PDB ID: 3LHDDownload
MMDB ID: 82204
PDB Deposition Date: 2010/1/22
Updated in MMDB: 2010/10
Experimental Method:
x-ray diffraction
Resolution: 2.59  Å
Source Organism:
Similar Structures:
Biological Unit for 3LHD: tetrameric; determined by author and by software (PISA)
Molecular Components in 3LHD
Label Count Molecule
Proteins (4 molecules)
4
Sam-dependent Methyltransferase, Putative(Gene symbol: PAB_RS02270)
Molecule annotation
Chemicals (4 molecules)
1
4
* Click molecule labels to explore molecular sequence information.

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