3KY9: Autoinhibited Vav1

Citation:
Abstract
Vav proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases. They control processes including T cell activation, phagocytosis, and migration of normal and transformed cells. We report the structure and biophysical and cellular analyses of the five-domain autoinhibitory element of Vav1. The catalytic Dbl homology (DH) domain of Vav1 is controlled by two energetically coupled processes. The DH active site is directly, but weakly, inhibited by a helix from the adjacent Acidic domain. This core interaction is strengthened 10-fold by contacts of the calponin homology (CH) domain with the Acidic, pleckstrin homology, and DH domains. This construction enables efficient, stepwise relief of autoinhibition: initial phosphorylation events disrupt the modulatory CH contacts, facilitating phosphorylation of the inhibitory helix and consequent GEF activation. Our findings illustrate how the opposing requirements of strong suppression of activity and rapid kinetics of activation can be achieved in multidomain systems.
PDB ID: 3KY9Download
MMDB ID: 80303
PDB Deposition Date: 2009/12/4
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 2.73  Å
Source Organism:
Similar Structures:
Biological Unit for 3KY9: monomeric; determined by author
Molecular Components in 3KY9
Label Count Molecule
Protein (1 molecule)
1
Proto-oncogene VAV(Gene symbol: VAV1)
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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