3KQI: Crystal Structure Of Phf2 Phd Domain Complexed With H3k4me3 Peptide

Citation:
Abstract
Distinct lysine methylation marks on histones create dynamic signatures deciphered by the "effector" modules, although the underlying mechanisms remain unclear. We identified the plant homeodomain- and Jumonji C domain-containing protein PHF2 as a novel histone H3K9 demethylase. We show in biochemical and crystallographic analyses that PHF2 recognizes histone H3K4 trimethylation through its plant homeodomain finger and that this interaction is essential for PHF2 occupancy and H3K9 demethylation at rDNA promoters. Our study provides molecular insights into the mechanism by which distinct effector domains within a protein cooperatively modulate the "cross-talk" of histone modifications.
PDB ID: 3KQIDownload
MMDB ID: 79799
PDB Deposition Date: 2009/11/17
Updated in MMDB: 2012/09
Experimental Method:
x-ray diffraction
Resolution: 1.78  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3KQI: dimeric; determined by author and by software (PISA)
Molecular Components in 3KQI
Label Count Molecule
Proteins (2 molecules)
1
PHD Finger Protein 2(Gene symbol: PHF2)
Molecule annotation
1
H3k4me3 Peptide(Gene symbol: HIST1H3A)
Molecule annotation
Chemicals (6 molecules)
1
2
2
1
3
1
4
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.