3K1L: Crystal Structure Of Fancl

Citation:
Abstract
The Fanconi anemia (FA) pathway is activated in response to DNA damage, leading to monoubiquitination of the substrates FANCI and FANCD2 by the FA core complex. Here we report the crystal structure of FANCL, the catalytic subunit of the FA core complex, at 3.2 A. The structure reveals an architecture fundamentally different from previous sequence-based predictions. The molecule is composed of an N-terminal E2-like fold, which we term the ELF domain, a novel double-RWD (DRWD) domain, and a C-terminal really interesting new gene (RING) domain predicted to facilitate E2 binding. Binding assays show that the DRWD domain, but not the ELF domain, is responsible for substrate binding.
PDB ID: 3K1LDownload
MMDB ID: 80128
PDB Deposition Date: 2009/9/28
Updated in MMDB: 2011/05
Experimental Method:
x-ray diffraction
Resolution: 3.2  Å
Source Organism:
Similar Structures:
Biological Unit for 3K1L: monomeric; determined by author
Molecular Components in 3K1L
Label Count Molecule
Protein (1 molecule)
1
Fancl
Molecule annotation
Chemicals (12 molecules)
1
2
2
1
3
1
4
8
* Click molecule labels to explore molecular sequence information.

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