3JU9: Crystal Structure Of A Lectin From Canavalia Brasiliensis Seed (conbr) Complexed With Alpha-aminobutyric Acid

Citation:
Abstract
Diocleinae lectins are highly homologous in their primary structure which features metal binding sites and a carbohydrate recognition domain (CRD). Differences in the biological activity of legume lectins have been widely investigated using hemagglutination inhibition assays, isothermal titration microcalorimetry and co-crystallization with mono- and oligosaccharides. Here we report a new lectin crystal structure (ConBr) extracted from seeds of Canavalia brasiliensis, predict dimannoside binding by docking, identify the alpha-aminobutyric acid (Abu) binding pocket and compare the CRD of ConBr to that of homologous lectins. Based on the hypothesis that the carbohydrate affinity of lectins depends on CRD configuration, the relationship between tridimensional structure and endothelial NO synthase activation was used to clarify differences in biological activity. Our study established a correlation between the position of CRD amino acid side chains and the stimulation of NO release from endothelium.
PDB ID: 3JU9Download
MMDB ID: 87125
PDB Deposition Date: 2009/9/14
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 3JU9: tetrameric; determined by author and by software (PISA)
Molecular Components in 3JU9
Label Count Molecule
Proteins (4 molecules)
4
Concanavalin-br
Molecule annotation
Chemicals (24 molecules)
1
4
2
4
3
4
4
4
5
8
* Click molecule labels to explore molecular sequence information.

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