3JRP: Sec13 With Nup145c (aa109-179) Insertion Blade

Nuclear pore complexes (NPCs) facilitate all nucleocytoplasmic transport. These massive protein assemblies are modular, with a stable structural scaffold supporting more dynamically attached components. The scaffold is made from multiple copies of the heptameric Y complex and the heteromeric Nic96 complex. We previously showed that members of these core subcomplexes specifically share an ACE1 fold with Sec31 of the COPII vesicle coat, and we proposed a lattice model for the NPC based on this commonality. Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84-Nup145C-Sec13 of the Y complex. The heterotypic ACE1 interaction of Nup84 and Nup145C is analogous to the homotypic ACE1 interaction of Sec31 that forms COPII lattice edge elements and is inconsistent with the alternative 'fence-like' NPC model. We construct a molecular model of the Y complex and compare the architectural principles of COPII and NPC lattices.
PDB ID: 3JRPDownload
MMDB ID: 77699
PDB Deposition Date: 2009/9/8
Updated in MMDB: 2009/11
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
Similar Structures:
Biological Unit for 3JRP: monomeric; determined by author and by software (PISA)
Molecular Components in 3JRP
Label Count Molecule
Protein (1 molecule)
Fusion Protein of Protein Transport Protein Sec13 and Nucleoporin Nup145
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB