3JCU: Cryo-em Structure Of Spinach Psii-lhcii Supercomplex At 3.2 Angstrom Resolution

Citation:
Abstract
During photosynthesis, the plant photosystem II core complex receives excitation energy from the peripheral light-harvesting complex II (LHCII). The pathways along which excitation energy is transferred between them, and their assembly mechanisms, remain to be deciphered through high-resolution structural studies. Here we report the structure of a 1.1-megadalton spinach photosystem II-LHCII supercomplex solved at 3.2 A resolution through single-particle cryo-electron microscopy. The structure reveals a homodimeric supramolecular system in which each monomer contains 25 protein subunits, 105 chlorophylls, 28 carotenoids and other cofactors. Three extrinsic subunits (PsbO, PsbP and PsbQ), which are essential for optimal oxygen-evolving activity of photosystem II, form a triangular crown that shields the Mn4CaO5-binding domains of CP43 and D1. One major trimeric and two minor monomeric LHCIIs associate with each core-complex monomer, and the antenna-core interactions are reinforced by three small intrinsic subunits (PsbW, PsbH and PsbZ). By analysing the closely connected interfacial chlorophylls, we have obtained detailed insights into the energy-transfer pathways between the antenna and core complexes.
PDB ID: 3JCUDownload
MMDB ID: 139444
PDB Deposition Date: 2016/3/10
Updated in MMDB: 2016/06
Experimental Method:
electron microscopy
Resolution: 3.2  Å
Source Organism:
Similar Structures:
Biological Unit for 3JCU: 50-meric; determined by author
Molecular Components in 3JCU
Label Count Molecule
Proteins (50 molecules)
2
Photosystem II Protein D1(Gene symbol: psbA)
Molecule annotation
2
Photosystem II Cp47 Reaction Center Protein(Gene symbol: psbB)
Molecule annotation
2
Photosystem II Cp43 Reaction Center Protein(Gene symbol: psbC)
Molecule annotation
2
Photosystem II D2 Protein(Gene symbol: psbD)
Molecule annotation
2
Cytochrome B559 Subunit Alpha(Gene symbol: psbE)
Molecule annotation
2
Cytochrome B559 Subunit Beta(Gene symbol: psbF)
Molecule annotation
6
Chlorophyll A-B Binding Protein, Chloroplastic
Molecule annotation
2
Photosystem II Reaction Center Protein H(Gene symbol: psbH)
Molecule annotation
2
Protein Photosystem II Reaction Center Protein I(Gene symbol: psbI)
Molecule annotation
2
Photosystem II Reaction Center Protein J(Gene symbol: psbJ)
Molecule annotation
2
Photosystem II Reaction Center Protein K(Gene symbol: psbK)
Molecule annotation
2
Protein Photosystem II Reaction Center Protein L(Gene symbol: psbL)
Molecule annotation
2
Photosystem II Reaction Center Protein M(Gene symbol: psbM)
Molecule annotation
2
Oxygen-evolving Enhancer Protein 1, Chloroplastic
Molecule annotation
2
Oxygen-evolving Enhancer Protein 2, Chloroplastic
Molecule annotation
2
Oxygen-evolving Enhancer Protein 3, Chloroplastic
Molecule annotation
2
Chlorophyll A-B Binding Protein 29 KD (Cp29)
Molecule annotation
2
Chlorophyll A-B Binding Protein 26 KD (Cp26)
Molecule annotation
2
Photosystem II Reaction Center Protein TC(Gene symbol: psbT)
Molecule annotation
2
Photosystem II Reaction Center TN Protein
Molecule annotation
2
Photosystem II Reaction Center W Protein, Chloroplastic
Molecule annotation
2
Photosystem II Reaction Center X Protein
Molecule annotation
2
Photosystem II Reaction Center Protein Z(Gene symbol: psbZ)
Molecule annotation
Chemicals (322 molecules)
1
2
2
2
3
4
4
156
5
4
6
20
7
6
8
10
9
8
10
2
11
2
12
18
13
2
14
50
15
18
16
8
17
10
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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