3JA5: Genome and RdRp structure within the capsid of no-transcribing cypovirus

Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures.
PDB ID: 3JA5Download
MMDB ID: 133618
PDB Deposition Date: 2015/4/21
Updated in MMDB: 2015/10
Experimental Method:
electron microscopy
Resolution: 12  Å
Source Organism:
Similar Structures:
Biological Unit for 3JA5: hexameric; determined by author
Molecular Components in 3JA5
Label Count Molecule
Proteins (6 molecules)
RNA-dependent RNA Polymerase
Molecule annotation
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Citing MMDB