3J36: Tetracycline Resistance Protein Tet(o) Bound To The Ribosome

Citation:
Abstract
Tetracycline resistance protein Tet(O), which protects the bacterial ribosome from binding the antibiotic tetracycline, is a translational GTPase with significant similarity in both sequence and structure to the elongation factor EF-G. Here, we present an atomic model of the Tet(O)-bound 70S ribosome based on our cryo-electron microscopic reconstruction at 9.6-A resolution. This atomic model allowed us to identify the Tet(O)-ribosome binding sites, which involve three characteristic loops in domain 4 of Tet(O). Replacements of the three amino-acid tips of these loops by a single glycine residue result in loss of Tet(O)-mediated tetracycline resistance. On the basis of these findings, the mechanism of Tet(O)-mediated tetracycline resistance can be explained in molecular detail.
MMDB ID: 108937
PDB Deposition Date: 2013/2/25
Updated in MMDB: 2013/04
Experimental Method:
electron microscopy
Resolution: 9.8  Å
Source Organism:
Escherichia coli
Similar Structures:
Merged PDB IDs:3J36 3J37
Biological Unit for 3J36: 58-meric
Molecular Components in 3J36
Label Count Molecule
Proteins (53 molecules)
1
30S Ribosomal Protein S10
Molecule annotation
1
30S Ribosomal Protein S11
Molecule annotation
1
30S Ribosomal Protein S12
Molecule annotation
1
30S Ribosomal Protein S13
Molecule annotation
1
30S Ribosomal Protein S14
Molecule annotation
1
30S Ribosomal Protein S15
Molecule annotation
1
30S Ribosomal Protein S16
Molecule annotation
1
30S Ribosomal Protein S17
Molecule annotation
1
30S Ribosomal Protein S18
Molecule annotation
1
30S Ribosomal Protein S19
Molecule annotation
1
30S Ribosomal Protein S2
Molecule annotation
1
30S Ribosomal Protein S20
Molecule annotation
1
30S Ribosomal Protein S21
Molecule annotation
1
30S Ribosomal Protein S3
Molecule annotation
1
30S Ribosomal Protein S4
Molecule annotation
1
30S Ribosomal Protein S5
Molecule annotation
1
30S Ribosomal Protein S6
Molecule annotation
1
30S Ribosomal Protein S7
Molecule annotation
1
30S Ribosomal Protein S8
Molecule annotation
1
30S Ribosomal Protein S9
Molecule annotation
1
Tetracycline Resistance Protein Teto
Molecule annotation
1
50S Ribosomal Protein L1
Molecule annotation
1
50S Ribosomal Protein L10
Molecule annotation
1
50S Ribosomal Protein L11
Molecule annotation
1
50S Ribosomal Protein L13
Molecule annotation
1
50S Ribosomal Protein L14
Molecule annotation
1
50S Ribosomal Protein L15
Molecule annotation
1
50S Ribosomal Protein L16
Molecule annotation
1
50S Ribosomal Protein L17
Molecule annotation
1
50S Ribosomal Protein L18
Molecule annotation
1
50S Ribosomal Protein L19
Molecule annotation
1
50S Ribosomal Protein L2
Molecule annotation
1
50S Ribosomal Protein L20
Molecule annotation
1
50S Ribosomal Protein L21
Molecule annotation
1
50S Ribosomal Protein L22
Molecule annotation
1
50S Ribosomal Protein L23
Molecule annotation
1
50S Ribosomal Protein L24
Molecule annotation
1
50S Ribosomal Protein L25
Molecule annotation
1
50S Ribosomal Protein L27
Molecule annotation
1
50S Ribosomal Protein L28
Molecule annotation
1
50S Ribosomal Protein L29
Molecule annotation
1
50S Ribosomal Protein L3
Molecule annotation
1
50S Ribosomal Protein L30
Molecule annotation
1
50S Ribosomal Protein L31
Molecule annotation
1
50S Ribosomal Protein L32
Molecule annotation
1
50S Ribosomal Protein L33
Molecule annotation
1
50S Ribosomal Protein L34
Molecule annotation
1
50S Ribosomal Protein L35
Molecule annotation
1
50S Ribosomal Protein L36
Molecule annotation
1
50S Ribosomal Protein L4
Molecule annotation
1
50S Ribosomal Protein L5
Molecule annotation
1
50S Ribosomal Protein L6
Molecule annotation
1
50S Ribosomal Protein L9
Molecule annotation
Nucleotides(5 molecules)
1
16S Ribosomal RNA
Molecule annotation
1
mRNA
Molecule annotation
1
P-trna
Molecule annotation
1
23S Ribosomal RNA
Molecule annotation
1
5S Ribosomal RNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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