3J2U: Kinesin-13 Klp10a Hd In Complex With Cs-tubulin And A Microtubule

To elucidate the structural basis of the mechanism of microtubule depolymerization by kinesin-13s, we analyzed complexes of tubulin and the Drosophila melanogaster kinesin-13 KLP10A by electron microscopy (EM) and fluorescence polarization microscopy. We report a nanometer-resolution (1.1 nm) cryo-EM three-dimensional structure of the KLP10A head domain (KLP10AHD) bound to curved tubulin. We found that binding of KLP10AHD induces a distinct tubulin configuration with displacement (shear) between tubulin subunits in addition to curvature. In this configuration, the kinesin-binding site differs from that in straight tubulin, providing an explanation for the distinct interaction modes of kinesin-13s with the microtubule lattice or its ends. The KLP10AHD-tubulin interface comprises three areas of interaction, suggesting a crossbow-type tubulin-bending mechanism. These areas include the kinesin-13 family conserved KVD residues, and as predicted from the crossbow model, mutating these residues changes the orientation and mobility of KLP10AHDs interacting with the microtubule.
PDB ID: 3J2UDownload
MMDB ID: 108040
PDB Deposition Date: 2013/1/10
Updated in MMDB: 2013/04
Experimental Method:
electron microscopy
Resolution: 10.8  Å
Source Organism:
Bos taurus
Similar Structures:
Biological Unit for 3J2U: 210-meric
Molecular Components in 3J2U
Label Count Molecule
Proteins (210 molecules)
Kinesin-like Protein Klp10a(Gene symbol: Klp10A)
Molecule annotation
Tubulin Alpha-1a Chain(Gene symbol: TUBA1A)
Molecule annotation
Tubulin Beta-2b Chain(Gene symbol: TUBB2B)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB