3J1X: A Refined Model Of The Prototypical Salmonella Typhimurium T3ss Basal Body Reveals The Molecular Basis For Its Assembly

Citation:
Abstract
The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a "basal body", a lock-nut structure spanning both bacterial membranes, and a "needle" that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism.
PDB ID: 3J1XDownload
MMDB ID: 110203
PDB Deposition Date: 2012/7/10
Updated in MMDB: 2013/05
Experimental Method:
electron microscopy
Source Organism:
Similar Structures:
Biological Unit for 3J1X: 24-meric; determined by author
Molecular Components in 3J1X
Label Count Molecule
Proteins (24 molecules)
24
Protein Prgh(Gene symbol: prgH)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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