3J1A: Hk97-Like Fold Fitted Into 3d Reconstruction Of Bacteriophage Cw02

Citation:
Abstract
Halophage CW02 infects a Salinivibrio costicola-like bacterium, SA50, isolated from the Great Salt Lake. Following isolation, cultivation, and purification, CW02 was characterized by DNA sequencing, mass spectrometry, and electron microscopy. A conserved module of structural genes places CW02 in the T7 supergroup, members of which are found in diverse aquatic environments, including marine and freshwater ecosystems. CW02 has morphological similarities to viruses of the Podoviridae family. The structure of CW02, solved by cryogenic electron microscopy and three-dimensional reconstruction, enabled the fitting of a portion of the bacteriophage HK97 capsid protein into CW02 capsid density, thereby providing additional evidence that capsid proteins of tailed double-stranded DNA phages have a conserved fold. The CW02 capsid consists of bacteriophage lambda gpD-like densities that likely contribute to particle stability. Turret-like densities were found on icosahedral vertices and may represent a unique adaptation similar to what has been seen in other extremophilic viruses that infect archaea, such as Sulfolobus turreted icosahedral virus and halophage SH1.
PDB ID: 3J1ADownload
MMDB ID: 100026
PDB Deposition Date: 2012/1/31
Updated in MMDB: 2012/08
Experimental Method:
electron microscopy
Resolution: 16  Å
Source Organism:
Similar Structures:
Biological Unit for 3J1A: 420-meric
Molecular Components in 3J1A
Label Count Molecule
Proteins (420 molecules)
420
Capsid Protein
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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