3J0Q: Core Of Mammalian 80s Pre-Ribosome In Complex With Trnas Fitted To A 10.6a Cryo-Em Map: Rotated Pre State 2

Citation:
Abstract
Although the structural core of the ribosome is conserved in all kingdoms of life, eukaryotic ribosomes are significantly larger and more complex than their bacterial counterparts. The extent to which these differences influence the molecular mechanism of translation remains elusive. Multiparticle cryo-electron microscopy and single-molecule FRET investigations of the mammalian pretranslocation complex reveal spontaneous, large-scale conformational changes, including an intersubunit rotation of the ribosomal subunits. Through structurally related processes, tRNA substrates oscillate between classical and at least two distinct hybrid configurations facilitated by localized changes in their L-shaped fold. Hybrid states are favored within the mammalian complex. However, classical tRNA positions can be restored by tRNA binding to the E site or by the eukaryotic-specific antibiotic and translocation inhibitor cycloheximide. These findings reveal critical distinctions in the structural and energetic features of bacterial and mammalian ribosomes, providing a mechanistic basis for divergent translation regulation strategies and species-specific antibiotic action.
PDB ID: 3J0QDownload
MMDB ID: 95024
PDB Deposition Date: 2011/10/11
Updated in MMDB: 2011/11
Experimental Method:
electron microscopy
Resolution: 10.6  Å
Source Organism:
Similar Structures:
Biological Unit for 3J0Q: 21-meric; determined by author
Molecular Components in 3J0Q
Label Count Molecule
Proteins (6 molecules)
1
Ribosomal Protein S15
Molecule annotation
1
Ribosomal Protein S23
Molecule annotation
1
Ribosomal Protein S30
Molecule annotation
1
Ribosomal Protein L10a
Molecule annotation
1
Ribosomal Protein L10
Molecule annotation
1
Ribosomal Protein L11
Molecule annotation
Nucleotides(14 molecules)
1
40S Ribosomal RNA Fragment
Molecule annotation
1
40S Ribosomal RNA Fragment
Molecule annotation
1
40S Ribosomal RNA Fragment
Molecule annotation
1
40S Ribosomal RNA Fragment
Molecule annotation
1
40S Ribosomal RNA Fragment
Molecule annotation
1
40S Ribosomal RNA Fragment
Molecule annotation
1
40S Ribosomal RNA Fragment
Molecule annotation
1
60S Ribosomal RNA Fragment
Molecule annotation
1
60S Ribosomal RNA Fragment
Molecule annotation
1
60S Ribosomal RNA Fragment
Molecule annotation
1
60S Ribosomal RNA Fragment
Molecule annotation
2
tRNA
Molecule annotation
1
mRNA Fragment
Molecule annotation
1
mRNA Fragment
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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