3IZQ: Structure Of The Dom34-Hbs1-Gdpnp Complex Bound To A Translating Ribosome

No-go decay (NGD) is a mRNA quality-control mechanism in eukaryotic cells that leads to degradation of mRNAs stalled during translational elongation. The key factors triggering NGD are Dom34 and Hbs1. We used cryo-EM to visualize NGD intermediates resulting from binding of the Dom34-Hbs1 complex to stalled ribosomes. At subnanometer resolution, all domains of Dom34 and Hbs1 were identified, allowing the docking of crystal structures and homology models. Moreover, the close structural similarity of Dom34 and Hbs1 to eukaryotic release factors (eRFs) enabled us to propose a model for the ribosome-bound eRF1-eRF3 complex. Collectively, our data provide structural insights into how stalled mRNA is recognized on the ribosome and how the eRF complex can simultaneously recognize stop codons and catalyze peptide release.
PDB ID: 3IZQDownload
MMDB ID: 90929
PDB Deposition Date: 2010/11/30
Updated in MMDB: 2011/10
Experimental Method:
electron microscopy
Resolution: 9.5  Å
Source Organism:
Similar Structures:
Biological Unit for 3IZQ: dimeric; determined by author
Molecular Components in 3IZQ
Label Count Molecule
Proteins (2 molecules)
Protein Dom34(Gene symbol: DOM34)
Molecule annotation
Elongation Factor 1 Alpha-like Protein(Gene symbol: HBS1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB