3HUW: Structure Of Ef-p Bound To The 70s Ribosome; This File Contains The 30s Subunit, Mrna, P-site Trna And Ef-p For Molecule I

Citation:
Abstract
Elongation factor P (EF-P) is an essential protein that stimulates the formation of the first peptide bond in protein synthesis. Here we report the crystal structure of EF-P bound to the Thermus thermophilus 70S ribosome along with the initiator transfer RNA N-formyl-methionyl-tRNA(i) (fMet-tRNA(i)(fMet)) and a short piece of messenger RNA (mRNA) at a resolution of 3.5 angstroms. EF-P binds to a site located between the binding site for the peptidyl tRNA (P site) and the exiting tRNA (E site). It spans both ribosomal subunits with its amino-terminal domain positioned adjacent to the aminoacyl acceptor stem and its carboxyl-terminal domain positioned next to the anticodon stem-loop of the P site-bound initiator tRNA. Domain II of EF-P interacts with the ribosomal protein L1, which results in the largest movement of the L1 stalk that has been observed in the absence of ratcheting of the ribosomal subunits. EF-P facilitates the proper positioning of the fMet-tRNA(i)(fMet) for the formation of the first peptide bond during translation initiation.
MMDB ID: 99484
PDB Deposition Date: 2009/6/15
Updated in MMDB: 2012/08
Experimental Method:
x-ray diffraction
Resolution: 3.1  Å
Source Organism:
Thermus thermophilus HB8
Similar Structures:
Merged PDB IDs:3HUW 3HUX 3HUY 3HUZ
Asymmetric Unit for 3HUW: 112-meric
Molecular Components in 3HUW
Label Count Molecule
Proteins (102 molecules)
2
30S Ribosomal Protein S2
Molecule annotation
2
30S Ribosomal Protein S3
Molecule annotation
2
30S Ribosomal Protein S4
Molecule annotation
2
30S Ribosomal Protein S5
Molecule annotation
2
30S Ribosomal Protein S6
Molecule annotation
2
30S Ribosomal Protein S7
Molecule annotation
2
30S Ribosomal Protein S8
Molecule annotation
2
30S Ribosomal Protein S9
Molecule annotation
2
30S Ribosomal Protein S10
Molecule annotation
2
30S Ribosomal Protein S11
Molecule annotation
2
30S Ribosomal Protein S12
Molecule annotation
2
30S Ribosomal Protein S13
Molecule annotation
2
30S Ribosomal Protein S14
Molecule annotation
2
30S Ribosomal Protein S15
Molecule annotation
2
30S Ribosomal Protein S16
Molecule annotation
2
30S Ribosomal Protein S17
Molecule annotation
2
30S Ribosomal Protein S18
Molecule annotation
2
30S Ribosomal Protein S19
Molecule annotation
2
30S Ribosomal Protein S20
Molecule annotation
2
30S Ribosomal Protein THX
Molecule annotation
2
Elongation Factor P
Molecule annotation
2
50S Ribosomal Protein L1
Molecule annotation
2
50S Ribosomal Protein L2
Molecule annotation
2
50S Ribosomal Protein L3
Molecule annotation
2
50S Ribosomal Protein L4
Molecule annotation
2
50S Ribosomal Protein L5
Molecule annotation
2
50S Ribosomal Protein L6
Molecule annotation
2
50S Ribosomal Protein L9
Molecule annotation
2
50S Ribosomal Protein L13
Molecule annotation
2
50S Ribosomal Protein L14
Molecule annotation
2
50S Ribosomal Protein L15
Molecule annotation
2
50S Ribosomal Protein L16
Molecule annotation
2
50S Ribosomal Protein L17
Molecule annotation
2
50S Ribosomal Protein L18
Molecule annotation
2
50S Ribosomal Protein L19
Molecule annotation
2
50S Ribosomal Protein L20
Molecule annotation
2
50S Ribosomal Protein L21
Molecule annotation
2
50S Ribosomal Protein L22
Molecule annotation
2
50S Ribosomal Protein L23
Molecule annotation
2
50S Ribosomal Protein L24
Molecule annotation
2
50S Ribosomal Protein L25
Molecule annotation
2
50S Ribosomal Protein L27
Molecule annotation
2
50S Ribosomal Protein L28
Molecule annotation
2
50S Ribosomal Protein L29
Molecule annotation
2
50S Ribosomal Protein L30
Molecule annotation
2
50S Ribosomal Protein L31
Molecule annotation
2
50S Ribosomal Protein L32
Molecule annotation
2
50S Ribosomal Protein L33
Molecule annotation
2
50S Ribosomal Protein L34
Molecule annotation
2
50S Ribosomal Protein L35
Molecule annotation
2
50S Ribosomal Protein L36
Molecule annotation
Nucleotides(5 molecules)
2
16S Ribosomal RNA
Molecule annotation
2
tRNA-met
Molecule annotation
2
RNA (5'-r(p*ap*ap*ap*up*g)-3')
Molecule annotation
2
23S Ribosomal RNA
Molecule annotation
2
5S Ribosomal RNA
Molecule annotation
Chemicals (1056 molecules)
1
1050
2
6
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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