3HKB: Tubulin: Rb3 Stathmin-Like Domain Complex

Structural changes occur in the alphabeta-tubulin heterodimer during the microtubule assembly/disassembly cycle. Their most prominent feature is a transition from a straight, microtubular structure to a curved structure. There is a broad range of small molecule compounds that disturbs the microtubule cycle, a class of which targets the colchicine-binding site and prevents microtubule assembly. This class includes compounds with very different chemical structures, and it is presently unknown whether they prevent tubulin polymerization by the same mechanism. To address this issue, we have determined the structures of tubulin complexed with a set of such ligands and show that they interfere with several of the movements of tubulin subunits structural elements upon its transition from curved to straight. We also determined the structure of tubulin unliganded at the colchicine site; this reveals that a beta-tubulin loop (termed T7) flips into this site. As with colchicine site ligands, this prevents a helix which is at the interface with alpha-tubulin from stacking onto a beta-tubulin beta sheet as in straight protofilaments. Whereas in the presence of these ligands the interference with microtubule assembly gets frozen, by flipping in and out the beta-subunit T7 loop participates in a reversible way in the resistance to straightening that opposes microtubule assembly. Our results suggest that it thereby contributes to microtubule dynamic instability.
PDB ID: 3HKBDownload
MMDB ID: 76449
PDB Deposition Date: 2009/5/23
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 3.65  Å
Source Organism:
Rattus norvegicus
Similar Structures:
Biological Unit for 3HKB: pentameric; determined by author and by software (PISA)
Molecular Components in 3HKB
Label Count Molecule
Proteins (5 molecules)
Tubulin Alpha Chain
Molecule annotation
Tubulin Beta Chain
Molecule annotation
Stathmin-4(Gene symbol: Stmn4)
Molecule annotation
Chemicals (6 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB