3H5X: Crystal Structure Of 2'-Amino-2'-Deoxy-Cytidine-5'-Triphosphate Bound To Norovirus Gii Rna Polymerase

Citation:
Abstract
Crystal structures of a genogroup II.4 human norovirus polymerase bound to an RNA primer-template duplex and the substrate analogue 2'-amino-2'-deoxycytidine-5'-triphosphate have been determined to 1.8 A resolution. The alteration of the substrate-binding site that is required to accommodate the 2'-amino group leads to a rearrangement of the polymerase active site and a disruption of the coordination shells of the active-site metal ions. The mode of binding seen for 2'-amino-2'-deoxycytidine-5'-triphosphate suggests a novel molecular mechanism of inhibition that may be exploited for the design of inhibitors targeting viral RNA polymerases.
PDB ID: 3H5XDownload
MMDB ID: 72817
PDB Deposition Date: 2009/4/22
Updated in MMDB: 2012/09
Experimental Method:
x-ray diffraction
Resolution: 1.77  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3H5X: trimeric; determined by author and by software (PISA)
Molecular Components in 3H5X
Label Count Molecule
Protein (1 molecule)
1
RNA Dependent RNA Polymerase
Molecule annotation
Nucleotides(2 molecules)
1
5'-r(upgpcpcpcpgpgpg)-3'
Molecule annotation
1
5'-r(pupgpcpcpcpgpgpgpc)-3'
Molecule annotation
Chemicals (9 molecules)
1
4
2
1
3
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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