3GSL: Crystal Structure Of Psd-95 Tandem Pdz Domains 1 And 2

The interactions of the AMPA receptor (AMPAR) auxiliary subunit Stargazin with PDZ domain-containing scaffold proteins such as PSD-95 are critical for the synaptic stabilization of AMPARs. To investigate these interactions, we have developed biomimetic competing ligands that are assembled from two Stargazin-derived PSD-95/DLG/ZO-1 (PDZ) domain-binding motifs using 'click' chemistry. Characterization of the ligands in vitro and in a cellular FRET-based model revealed an enhanced affinity for the multiple PDZ domains of PSD-95 compared to monovalent peptides. In cultured neurons, the divalent ligands competed with transmembrane AMPAR regulatory protein (TARP) for the intracellular membrane-associated guanylate kinase resulting in increased lateral diffusion and endocytosis of surface AMPARs, while showing strong inhibition of synaptic AMPAR currents. This provides evidence for a model in which the TARP-containing AMPARs are stabilized at the synapse by engaging in multivalent interactions. In light of the prevalence of PDZ domain clusters, these new biomimetic chemical tools could find broad application for acutely perturbing multivalent complexes.
PDB ID: 3GSLDownload
MMDB ID: 85170
PDB Deposition Date: 2009/3/27
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 2.05  Å
Source Organism:
Similar Structures:
Biological Unit for 3GSL: monomeric; determined by author and by software (PISA)
Molecular Components in 3GSL
Label Count Molecule
Protein (1 molecule)
Disks Large Homolog 4(Gene symbol: Dlg4)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB