3G6Q: GR DNA binding domain:FKBP5 binding site complex-9

Genes are not simply turned on or off, but instead their expression is fine-tuned to meet the needs of a cell. How genes are modulated so precisely is not well understood. The glucocorticoid receptor (GR) regulates target genes by associating with specific DNA binding sites, the sequences of which differ between genes. Traditionally, these binding sites have been viewed only as docking sites. Using structural, biochemical, and cell-based assays, we show that GR binding sequences, differing by as little as a single base pair, differentially affect GR conformation and regulatory activity. We therefore propose that DNA is a sequence-specific allosteric ligand of GR that tailors the activity of the receptor toward specific target genes.
PDB ID: 3G6QDownload
MMDB ID: 71003
PDB Deposition Date: 2009/2/8
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.26  Å
Source Organism:
Rattus norvegicus
Similar Structures:
Biological Unit for 3G6Q: tetrameric; determined by author and by software (PISA)
Molecular Components in 3G6Q
Label Count Molecule
Proteins (2 molecules)
Glucocorticoid Receptor(Gene symbol: Nr3c1)
Molecule annotation
Nucleotides(2 molecules)
DNA (5'-d(*tp*ap*gp*ap*ap*cp*ap*gp*gp*gp*tp*gp*tp*tp*cp*t)-3')
Molecule annotation
DNA (5'-d(*ap*ap*gp*ap*ap*cp*ap*cp*cp*cp*tp*gp*tp*tp*cp*t)-3')
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB