3FAS: X-ray Structure of Iglur4 Flip Ligand-binding Core (S1s2) in Complex With (S)-glutamate at 1.40a Resolution

Citation:
Abstract
The alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) class of ionotropic glutamate receptors comprises four different subunits: iGluR1/iGluR2 and iGluR3/iGluR4 forming two subgroups. Three-dimensional structures have been reported only of the ligand-binding core of iGluR2. Here, we present two X-ray structures of a soluble construct of the R/G unedited flip splice variant of the ligand-binding core of iGluR4 (iGluR4(i)(R)-S1S2) in complex with glutamate or AMPA. Subtle, but important differences are found in the ligand-binding cavity between the two AMPA receptor subgroups at position 724 (Tyr in iGluR1/iGluR2 and Phe in iGluR3/iGluR4), which in iGluR4 may lead to displacement of a water molecule and hence points to the possibility to make subgroup specific ligands.
PDB ID: 3FASDownload
MMDB ID: 106346
PDB Deposition Date: 2008/11/18
Updated in MMDB: 2017/08
Experimental Method:
x-ray diffraction
Resolution: 1.4  Å
Source Organism:
Similar Structures:
Biological Unit for 3FAS: dimeric; determined by author and by software (PISA)
Molecular Components in 3FAS
Label Count Molecule
Proteins (2 molecules)
2
Glutamate Receptor 4(Gene symbol: Gria4)
Molecule annotation
Chemicals (19 molecules)
1
2
2
8
3
9
* Click molecule labels to explore molecular sequence information.

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