3F4D: Crystal Structure Of Organophosphorus Hydrolase From Geobacillus Stearothermophilus Strain 10

A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possesses higher OP-degrading activity than recently characterized PLLs, and it is extremely thermostable. GsP is active up to 100 degrees C with an energy of activation of 8.0 kcal/mol towards ethyl paraoxon, and it can withstand an incubation temperature of 60 degrees C for two days. In an attempt to understand the thermostability of PLLs, the X-ray structure of GsP was determined and compared to those of existing PLLs. Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs.
PDB ID: 3F4DDownload
MMDB ID: 76066
PDB Deposition Date: 2008/10/31
Updated in MMDB: 2009/09
Experimental Method:
x-ray diffraction
Resolution: 2.36  Å
Source Organism:
Similar Structures:
Biological Unit for 3F4D: dimeric; determined by author and by software (PISA)
Molecular Components in 3F4D
Label Count Molecule
Proteins (2 molecules)
Organophosphorus Hydrolase
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

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