3F27: Structure Of Sox17 Bound To Dna

Citation:
Abstract
Sox17 regulates endodermal lineage commitment and is thought to function antagonistically to the pluripotency determinant Sox2. To investigate the biochemical basis for the distinct functions of Sox2 and Sox17, we solved the crystal structure of the high mobility group domain of Sox17 bound to a DNA element derived from the Lama1 enhancer using crystals diffracting to 2.7 A resolution. Sox17 targets the minor groove and bends the DNA by approximately 80 degrees . The DNA architecture closely resembles the one seen for Sox2/DNA structures, suggesting that the degree of bending is conserved between both proteins and nucleotide substitutions have only marginal effects on the bending topology. Accordingly, affinities of Sox2 and Sox17 for the Lama1 element were found to be identical. However, when the Oct1 contact interface of Sox2 is compared with the corresponding region of Sox17, a significantly altered charge distribution is observed, suggesting differential co-factor recruitment that may explain their biological distinctiveness.
PDB ID: 3F27Download
MMDB ID: 70700
PDB Deposition Date: 2008/10/29
Updated in MMDB: 2012/10
Experimental Method:
x-ray diffraction
Resolution: 2.75  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3F27: trimeric; determined by author and by software (PISA)
Molecular Components in 3F27
Label Count Molecule
Protein (1 molecule)
1
Transcription Factor Sox-17(Gene symbol: Sox17)
Molecule annotation
Nucleotides(2 molecules)
1
DNA (5'- D(*dgp*dtp*dcp*dtp*dcp*dtp*dap*dtp*dtp*dgp*dtp*dcp*dcp*dtp*dgp*dg)- 3')
Molecule annotation
1
DNA (5'- D(*dcp*dcp*dap*dgp*dgp*dap*dcp*dap*dap*dtp*dap*dgp*dap*dgp*dap*dc)- 3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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