3F1I: Human Escrt-0 Core Complex

The human Hrs and STAM proteins comprise the ESCRT-0 complex, which sorts ubiquitinated cell surface receptors to lysosomes for degradation. Here we report a model for the complete ESCRT-0 complex based on the crystal structure of the Hrs-STAM core complex, previously solved domain structures, hydrodynamic measurements, and Monte Carlo simulations. ESCRT-0 expressed in insect cells has a hydrodynamic radius of RH = 7.9 nm and is a 1:1 heterodimer. The 2.3 Angstroms crystal structure of the ESCRT-0 core complex reveals two domain-swapped GAT domains and an antiparallel two-stranded coiled-coil, similar to yeast ESCRT-0. ESCRT-0 typifies a class of biomolecular assemblies that combine structured and unstructured elements, and have dynamic and open conformations to ensure versatility in target recognition. Coarse-grained Monte Carlo simulations constrained by experimental RH values for ESCRT-0 reveal a dynamic ensemble of conformations well suited for diverse functions.
PDB ID: 3F1IDownload
MMDB ID: 70382
PDB Deposition Date: 2008/10/28
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 3F1I: trimeric; determined by author and by software (PISA)
Molecular Components in 3F1I
Label Count Molecule
Proteins (3 molecules)
Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate(Gene symbol: HGS)
Molecule annotation
Signal Transducing Adapter Molecule 1(Gene symbol: STAM)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB