3DOM: Crystal Structure Of The Complex Between Tfb5 And The C-terminal Domain Of Tfb2

Patients with the rare neurodevelopmental repair syndrome known as group A trichothiodystrophy (TTD-A) carry mutations in the gene encoding the p8 subunit of the transcription and DNA repair factor TFIIH. Here we describe the crystal structure of a minimal complex between Tfb5, the yeast ortholog of p8, and the C-terminal domain of Tfb2, the yeast p52 subunit of TFIIH. The structure revealed that these two polypeptides adopt the same fold, forming a compact pseudosymmetric heterodimer via a beta-strand addition and coiled coils interactions between terminal alpha-helices. Furthermore, Tfb5 protects a hydrophobic surface in Tfb2 from solvent, providing a rationale for the influence of p8 in the stabilization of p52 and explaining why mutations that weaken p8-p52 interactions lead to a reduced intracellular TFIIH concentration and a defect in nucleotide-excision repair, a common feature of TTD cells.
PDB ID: 3DOMDownload
MMDB ID: 66200
PDB Deposition Date: 2008/7/4
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
Similar Structures:
Biological Unit for 3DOM: dimeric; determined by author and by software (PISA)
Molecular Components in 3DOM
Label Count Molecule
Proteins (2 molecules)
RNA Polymerase II Transcription Factor B Subunit 2(Gene symbol: TFB2)
Molecule annotation
RNA Polymerase II Transcription Factor B Subunit 5(Gene symbol: TFB5)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB