3D96: Crystal Structure Of The R132k:y134f Mutant Of Apo-cellular Retinoic Acid Binding Protein Type Ii At 1.71 Angstroms Resolution

The structural integrity of cellular retinoic acid-binding protein II (CRABPII) has been investigated using the crystal structures of CRABPII mutants. The overall fold was well maintained by these CRABPII mutants, each of which carried multiple different mutations. A water-mediated network is found to be present across the large binding cavity, extending from Arg111 deep inside the cavity to the alpha2 helix at its entrance. This chain of interactions acts as a ;pillar' that maintains the integrity of the protein. The disruption of the water network upon loss of Arg111 leads to decreased structural integrity of the protein. A water-mediated network can be re-established by introducing the hydrophilic Glu121 inside the cavity, which results in a rigid protein with the alpha2 helix adopting an altered conformation compared with wild-type CRABPII.
PDB ID: 3D96Download
MMDB ID: 65632
PDB Deposition Date: 2008/5/26
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 1.71  Å
Source Organism:
Similar Structures:
Biological Unit for 3D96: monomeric; determined by author and by software (PISA)
Molecular Components in 3D96
Label Count Molecule
Protein (1 molecule)
Cellular Retinoic Acid-binding Protein 2(Gene symbol: CRABP2)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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