3D7F: A High Resolution Crystal Structure Of Human Glutamate Carboxypeptidase Ii (Gcpii) In A Complex With Dcit, A Urea-Based Inhibitor

Citation:
Abstract
Urea-based, low molecular weight ligands of glutamate carboxypeptidase II (GCPII) have demonstrated efficacy in various models of neurological disorders and can serve as imaging agents for prostate cancer. To enhance further development of such compounds, we determined X-ray structures of four complexes between human GCPII and urea-based inhibitors at high resolution. All ligands demonstrate an invariant glutarate moiety within the S1' pocket of the enzyme. The ureido linkage between P1 and P1' inhibitor sites interacts with the active-site Zn(1)(2+) ion and the side chains of Tyr552 and His553. Interactions within the S1 pocket are defined primarily by a network of hydrogen bonds between the P1 carboxylate group of the inhibitors and the side chains of Arg534, Arg536, and Asn519. Importantly, we have identified a hydrophobic pocket accessory to the S1 site that can be exploited for structure-based design of novel GCPII inhibitors with increased lipophilicity.
PDB ID: 3D7FDownload
MMDB ID: 68791
PDB Deposition Date: 2008/5/21
Updated in MMDB: 2008/12
Experimental Method:
x-ray diffraction
Resolution: 1.54  Å
Source Organism:
Similar Structures:
Biological Unit for 3D7F: dimeric; determined by author and by software (PISA)
Molecular Components in 3D7F
Label Count Molecule
Proteins (2 molecules)
2
Glutamate Carboxypeptidase 2(Gene symbol: FOLH1)
Molecule annotation
Chemicals (30 molecules)
1
18
2
2
3
4
4
2
5
2
6
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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