3D2Y: Complex Of The N-Acetylmuramyl-L-Alanine Amidase Amid From E.Coli With The Substrate Anhydro-N-Acetylmuramic Acid-L-Ala-D-Gamma-Glu-L-Lys

Citation:
Abstract
AmiD is the fifth identified N-acetylmuramoyl-L-alanine zinc amidase of Escherichia coli. This periplasmic lipoprotein is anchored in the outer membrane and has a broad specificity. AmiD is capable of cleaving the intact peptidoglycan (PG) as well as soluble fragments containing N-acetylmuramic acid regardless of the presence of an anhydro form or not, unlike the four other amidases, AmiA, AmiB, AmiC, and AmpD, which have some specificity. AmiD function is, however, not clearly established but it could be part of the enzymatic machinery involved in the PG turnover in E. coli. We solved three structures of the E. coli zinc amidase AmiD devoid of its lipidic anchorage: the holoenzyme, the apoenzyme in complex with the substrate anhydro-N-acetylmuramic-acid-L-Ala-gamma-d-Glu-L-Lys, and the holoenzyme in complex with the L-Ala-gamma-D-Glu-L-Lys peptide, the product of the hydrolysis of this substrate by AmiD. The AmiD structure shows a relatively flexible N-terminal extension that allows an easy reach of the PG by the enzyme inserted into the outer membrane. The C-terminal domain provides a potential extended geometrical complementarity to the substrate. AmiD shares a common fold with AmpD, the bacteriophage T7 lysozyme, and the PG recognition proteins, which are receptor proteins involved in the innate immune responses of a wide range of organisms. Analysis of the different structures reveals the similarity between the catalytic mechanism of zinc amidases of the AmiD family and the thermolysin-related zinc peptidases.
PDB ID: 3D2YDownload
MMDB ID: 73287
PDB Deposition Date: 2008/5/9
Updated in MMDB: 2011/08
Experimental Method:
x-ray diffraction
Resolution: 1.75  Å
Source Organism:
Escherichia coli str. K-12 substr. MG1655
Similar Structures:
Biological Unit for 3D2Y: dimeric; determined by author
Molecular Components in 3D2Y
Label Count Molecule
Proteins (2 molecules)
1
N-acetylmuramoyl-l-alanine Amidase Amid(Gene symbol: amiD)
Molecule annotation
1
Anhydro-n-acetylmuramic Acid-l-ala-d-gamma-glu-l-lys
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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