3D2D: Structure Of Berberine Bridge Enzyme In Complex With (S)-Reticuline

Citation:
Abstract
Berberine bridge enzyme catalyzes the conversion of (S)-reticuline to (S)-scoulerine by formation of a carbon-carbon bond between the N-methyl group and the phenolic ring. We elucidated the structure of berberine bridge enzyme from Eschscholzia californica and determined the kinetic rates for three active site protein variants. Here we propose a catalytic mechanism combining base-catalyzed proton abstraction with concerted carbon-carbon coupling accompanied by hydride transfer from the N-methyl group to the N5 atom of the FAD cofactor.
PDB ID: 3D2DDownload
MMDB ID: 67622
PDB Deposition Date: 2008/5/8
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Similar Structures:
Biological Unit for 3D2D: monomeric; determined by author and by software (PISA)
Molecular Components in 3D2D
Label Count Molecule
Protein (1 molecule)
1
Berberine Bridge-forming Enzyme
Molecule annotation
Chemicals (9 molecules)
1
3
2
4
3
1
4
1
* Click molecule labels to explore molecular sequence information.

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