3D0Q: Crystal Structure Of Calg3 From Micromonospora Echinospora Determined In Space Group I222

Citation:
Abstract
The enediyne antibiotic calicheamicin (CLM) gamma(1)(I) is a prominent antitumor agent that is targeted to DNA by a novel aryltetrasaccharide comprised of an aromatic unit and four unusual carbohydrates. Herein we report the heterologous expression and the biochemical characterization of the two "internal" glycosyltransferases CalG3 and CalG2 and the structural elucidation of an enediyne glycosyltransferase (CalG3). In conjunction with the previous characterization of the "external" CLM GTs CalG1 and CalG4, this study completes the functional assignment of all four CLM GTs, extends the utility of enediyne GT-catalyzed reaction reversibility, and presents conclusive evidence of a sequential glycosylation pathway in CLM biosynthesis. This work also reveals the common GT-B structural fold can now be extended to include enediyne GTs.
PDB ID: 3D0QDownload
MMDB ID: 65137
PDB Deposition Date: 2008/5/2
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 2.79  Å
Source Organism:
Similar Structures:
Biological Unit for 3D0Q: dimeric; determined by author and by software (PISA)
Molecular Components in 3D0Q
Label Count Molecule
Proteins (2 molecules)
2
Protein Calg3
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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