3CQC: Nucleoporin Nup107/nup133 Interaction Complex

Nuclear pore complexes (NPCs) are 40-60 MDa protein assemblies embedded in the nuclear envelope of eukaryotic cells. NPCs exclusively mediate all transport between cytoplasm and nucleus. The nucleoporins that build the NPC are arranged in a stable core of module-like subcomplexes with eight-fold rotational symmetry. To gain insight into the intricate assembly of the NPC, we have solved the crystal structure of a protein complex between two nucleoporins, human Nup107 and Nup133. Both proteins form elongated structures that interact tightly via a compact interface in tail-to-tail fashion. Additional experiments using structure-guided mutants show that Nup107 is the critical anchor for Nup133 to the NPC, positioning Nup133 at the periphery of the NPC. The significant topological differences between Nup107 and Nup133 suggest that *-helical nucleoporin domains of the NPC scaffold fall in different classes and fulfill largely nonredundant functions.
PDB ID: 3CQCDownload
MMDB ID: 65266
PDB Deposition Date: 2008/4/2
Updated in MMDB: 2009/07
Experimental Method:
x-ray diffraction
Resolution: 2.53  Å
Source Organism:
Similar Structures:
Biological Unit for 3CQC: dimeric; determined by software (PISA)
Molecular Components in 3CQC
Label Count Molecule
Proteins (2 molecules)
Nuclear Pore Complex Protein Nup107(Gene symbol: NUP107)
Molecule annotation
Nuclear Pore Complex Protein Nup133(Gene symbol: NUP133)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB