3C7N: Structure Of The Hsp110:hsc70 Nucleotide Exchange Complex

Citation:
Abstract
Hsp70s mediate protein folding, translocation, and macromolecular complex remodeling reactions. Their activities are regulated by proteins that exchange ADP for ATP from the nucleotide-binding domain (NBD) of the Hsp70. These nucleotide exchange factors (NEFs) include the Hsp110s, which are themselves members of the Hsp70 family. We report the structure of an Hsp110:Hsc70 nucleotide exchange complex. The complex is characterized by extensive protein:protein interactions and symmetric bridging interactions between the nucleotides bound in each partner protein's NBD. An electropositive pore allows nucleotides to enter and exit the complex. The role of nucleotides in complex formation and dissociation, and the effects of the protein:protein interactions on nucleotide exchange, can be understood in terms of the coupled effects of the nucleotides and protein:protein interactions on the open-closed isomerization of the NBDs. The symmetrical interactions in the complex may model other Hsp70 family heterodimers in which two Hsp70s reciprocally act as NEFs.
PDB ID: 3C7NDownload
MMDB ID: 64684
PDB Deposition Date: 2008/2/7
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 3.12  Å
Source Organism:
Bos taurus
Similar Structures:
Biological Unit for 3C7N: dimeric; determined by author and by software (PISA)
Molecular Components in 3C7N
Label Count Molecule
Proteins (2 molecules)
1
Heat Shock Protein Homolog Sse1(Gene symbol: SSE1)
Molecule annotation
1
Heat Shock Cognate(Gene symbol: HSPA8)
Molecule annotation
Chemicals (12 molecules)
1
2
2
4
3
1
4
2
5
3
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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