3C1V: The 1.5 A Crystal structure of Ca2+-bound S100A4

Citation:
Abstract
S100A4 takes part in control of tumour cell migration and contributes to metastatic spread in in vivo models. In the active dimeric Ca(2+)-bound state it interacts with multiple intracellular targets. Conversely, oligomeric forms of S100A4 are linked with the extracellular function of this protein. We report the 1.5A X-ray crystal structure of Ca(2+)-bound S100A4 and use it to identify the residues involved in target recognition and to derive a model of the oligomeric state. We applied stopped-flow analysis of tyrosine fluorescence to derive kinetics of S100A4 activation by Ca(2+) (k(on)=3.5 microM(-1)s(-1), k(off)=20s(-1)).
PDB ID: 3C1VDownload
MMDB ID: 63529
PDB Deposition Date: 2008/1/24
Updated in MMDB: 2009/07
Experimental Method:
x-ray diffraction
Resolution: 1.5  Å
Source Organism:
Similar Structures:
Biological Unit for 3C1V: dimeric; determined by author and by software (PISA)
Molecular Components in 3C1V
Label Count Molecule
Proteins (2 molecules)
2
Protein S100-a4(Gene symbol: S100A4)
Molecule annotation
Chemicals (4 molecules)
1
4
* Click molecule labels to explore molecular sequence information.

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