3BSQ: Crystal Structure Of Human Kallikrein 7 Produced As A Secretion Protein In E.Coli

Citation:
Abstract
Human kallikrein 7, a major protease of human skin, has been synthesized directly in its native conformation in Escherichia coli by forcing the secretion of the newly synthesized polypeptide into the bacterial periplasm. The procedure yields a stable kallikrein 7 with highly specific activity that is inhibited efficiently by its specific inhibitor LEKTI domain 6. The protein was crystallized, and its three-dimensional structure was solved in the absence of protease inhibitors. The structure obtained agrees with that reported recently for human tissue kallikrein 7 crystallized in the presence of protease inhibitors from a preparation obtained in a baculovirus protein expression system. A model of the interaction between the protease and its inhibitor is proposed on the basis of both the three-dimensional structure of human tissue kallikrein 7 reported here and that of the LEKTI domain 6 solved previously by NMR.
PDB ID: 3BSQDownload
MMDB ID: 64181
PDB Deposition Date: 2007/12/26
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Similar Structures:
Biological Unit for 3BSQ: monomeric; determined by author and by software (PISA)
Molecular Components in 3BSQ
Label Count Molecule
Protein (1 molecule)
1
Kallikrein-7(Gene symbol: KLK7)
Molecule annotation
Chemicals (7 molecules)
1
7
* Click molecule labels to explore molecular sequence information.

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