3BRB: Crystal Structure Of Catalytic Domain Of The Proto-Oncogene Tyrosine- Protein Kinase Mer In Complex With Adp

Citation:
Abstract
The mammalian ortholog of the retroviral oncogene v-Eyk, and a receptor tyrosine kinase upstream of antiapoptotic and transforming signals, Mer (MerTK) is a mediator of the phagocytic process, being involved in retinal and immune cell clearance and platelet aggregation. Mer knockout mice are viable and are protected from epinephrine-induced pulmonary thromboembolism and ferric chloride-induced thrombosis. Mer overexpression, on the other hand, is associated with numerous carcinomas. Although Mer adaptor proteins and signaling pathways have been identified, it remains unclear how Mer initiates phagocytosis. When bound to its nucleotide cofactor, the high-resolution structure of Mer shows an autoinhibited alphaC-Glu-out conformation with insertion of an activation loop residue into the active site. Mer complexed with compound-52 (C52: 2-(2-hydroxyethylamino)-6-(3-chloroanilino)-9-isopropylpurine), a ligand identified from a focused library, retains its DFG-Asp-in and alphaC-Glu-out conformation, but acquires other conformational changes. The alphaC helix and DFGL region is closer to the hinge region and the ethanolamine moiety of C52 binds in the groove formed between Leu593 and Val601 of the P-loop, causing a compression of the active site pocket. These conformational states reveal the mechanisms of autoinhibition, the pathophysiological basis of disease-causing mutations, and a platform for the development of chemical probes.
PDB ID: 3BRBDownload
MMDB ID: 62066
PDB Deposition Date: 2007/12/21
Updated in MMDB: 2012/09
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 3BRB: monomeric; determined by author and by software (PISA)
Molecular Components in 3BRB
Label Count Molecule
Protein (1 molecule)
1
Proto-oncogene Tyrosine-protein Kinase MER(Gene symbol: MERTK)
Molecule annotation
Chemicals (6 molecules)
1
1
2
1
3
3
4
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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