3BQ0: Pre-insertion Binary Complex Of Dbh Dna Polymerase

Citation:
Abstract
Dbh is a Y family translesion DNA polymerase that accurately bypasses some damaged forms of deoxyguanosine, but also generates single-base deletion errors at frequencies of up to 50%, in specific hot spot sequences. We describe preinsertion binary, insertion ternary, and postinsertion binary crystal structures of Dbh synthesizing DNA after making a single-base deletion. The skipped template base adopts an extrahelical conformation stabilized by interactions with the C-terminal domain of the enzyme. DNA translocation and positioning of the next templating base at the active site, with space opposite to accommodate incoming nucleotide, occur independently of nucleotide binding, incorporation, and pyrophosphate release. We also show that Dbh creates single-base deletions more rapidly when the skipped base is located two or three bases upstream of the nascent base pair than when it is directly adjacent to the templating base, indicating that Dbh predominantly creates single-base deletions by template slippage rather than by dNTP-stabilized misalignment.
PDB ID: 3BQ0Download
MMDB ID: 63711
PDB Deposition Date: 2007/12/19
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3BQ0: trimeric; determined by author and by software (PISA)
Molecular Components in 3BQ0
Label Count Molecule
Protein (1 molecule)
1
DNA Polymerase IV(Gene symbol: SACI_RS02640)
Molecule annotation
Nucleotides(2 molecules)
1
DNA (5'-d(*dgp*dap*dap*dgp*dcp*dcp*dgp*dgp*dcp*dg)-3')
Molecule annotation
1
DNA (5'- D(*dt*dtp*dcp*dcp*dgp*dcp*dcp*dcp*dgp*dgp*dcp*dtp*dtp*dcp*dc)-3')
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.