3BOP: Structure Of Mouse Beta-neurexin 2d4

Presynaptic neurexins (NRXs) bind to postsynaptic neuroligins (NLs) to form Ca(2+)-dependent complexes that bridge neural synapses. beta-NRXs bind NLs through their LNS domains, which contain a single site of alternative splicing (splice site 4) giving rise to two isoforms: +4 and Delta. We present crystal structures of the Delta isoforms of the LNS domains from beta-NRX1 and beta-NRX2, crystallized in the presence of Ca(2+) ions. The Ca(2+)-binding site is disordered in the beta-NRX2 structure, but the 1.7 A beta-NRX1 structure reveals a single Ca(2+) ion, approximately 12 A from the splice insertion site, with one coordinating ligand donated by a glutamic acid from an adjacent beta-NRX1 molecule. NMR studies of beta-NRX1+4 show that the insertion sequence is unstructured, and remains at least partially disordered in complex with NL. These results raise the possibility that beta-NRX insertion sequence 4 may function in roles independent of neuroligin binding.
PDB ID: 3BOPDownload
MMDB ID: 63289
PDB Deposition Date: 2007/12/17
Updated in MMDB: 2009/07
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Similar Structures:
Biological Unit for 3BOP: monomeric; determined by author and by software (PISA)
Molecular Components in 3BOP
Label Count Molecule
Protein (1 molecule)
Beta-neurexin 2D4
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB