3BNT: Crystal Structure Of The Homo Sapiens Mitochondrial Ribosomal Decoding Site In The Presence Of [co(nh3)6]cl3 (a1555g Mutant, Br-derivative)

The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or bacteria have been solved. The resting 'off' state of the mitochondrial wild-type A site is surprisingly different from that of the bacterial A site. The mitochondrial A1555G mutant has two types of the 'off' states; one is similar to the mitochondrial wild-type 'off' state and the other is similar to the bacterial 'off' state. Our present results indicate that the dynamics of the A site in bacteria and mitochondria are different, a property probably related to the small number of tRNAs used for decoding in mitochondria. Based on these structures, we propose a hypothesis for the molecular mechanism of non-syndromic hearing loss due to the mitochondrial A1555G mutation.
PDB ID: 3BNTDownload
MMDB ID: 65109
PDB Deposition Date: 2007/12/14
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Biological Unit for 3BNT: dimeric; determined by author and by software (PISA)
Molecular Components in 3BNT
Label Count Molecule
Nucleotide(1 molecule)
a Site of Human Mitochondrial Ribosome
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

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