3B2J: Iodide Derivative Of Human Lfabp

Citation:
Abstract
We report the use of anionic (I(-)), cationic (Ba(2+), Cd(2+)) and ionic mixtures (I(-) plus Ba(2+)) for derivatizing liver fatty acid binding protein (LFABP) crystals. Use of cationic and anionic salts in phasing experiments revealed distinct non-overlapping sites for these ions, suggesting exclusive binding regions on LFABP. Interestingly, cations of identical charge and valency (like Ba(2+) and Cd(2+)) bound to distinct pockets on the protein surface. Furthermore, a mixture of salts containing both I(-) and Ba(2+) was very useful in phasing experiments as these oppositely charged ions bound to different regions of LFABP. Our data therefore suggest that cationic and anionic salt mixtures like BaCl(2) with NH(4)I or salts like CdI, BaI where each ion has a significant anomalous signal for a given X-ray wavelength may be valuable reagents for phasing during structure determination.
PDB ID: 3B2JDownload
MMDB ID: 100623
PDB Deposition Date: 2011/8/3
Updated in MMDB: 2013/06
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Similar Structures:
Biological Unit for 3B2J: monomeric; determined by author and by software (PISA)
Molecular Components in 3B2J
Label Count Molecule
Protein (1 molecule)
1
Fatty Acid-binding Protein, Liver(Gene symbol: FABP1)
Molecule annotation
Chemicals (8 molecules)
1
2
2
6
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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